High resolution ordering of YAC contigs using extended chromatin and chromosomes
نویسندگان
چکیده
منابع مشابه
Ordered YAC clone contigs assigned to rice chromosomes 3 and 11.
Yeast artificial chromosome (YAC) clones were arranged on the positions of restriction fragment length polymorphism (RFLP) and sequence-tagged site (STS) markers already mapped on the high-resolution genetic maps of rice chromosomes 3 and 11. From a total of 416 and 242 YAC clones selected by colony/Southern hybridization and polymerase chain reaction (PCR) analysis, 238 and 135 YAC clones were...
متن کاملConstruction of YAC contigs on rice chromosome 5.
A physical map of rice chromosome 5 was constructed with yeast artificial chromosome (YAC) clones along a high-resolution molecular linkage map carrying 118 DNA markers distributed over 123.7 cM of genomic DNA. YAC clones have been identified by colony and Southern hybridization for 105 restriction fragment length polymorphism (RFLP) markers and by polymerase chain reaction (PCR) screening for ...
متن کاملChromatin and chromosomes
Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License
متن کاملAssignment of YAC clones spanning rice chromosomes 10 and 12.
Yeast artificial chromosome (YAC) clones were assigned on rice (Oryza saliva L. cv. Nipponbare) chromosomes 10 and 12 using DNA markers from our high-density linkage map. Out of 1,383 markers localized in this genetic map, 68 and 74 markers were located on chromosomes 10 and 12, respectively. Screening of the YAC genomic library was conducted by colony hybridization and Southern hybridization u...
متن کاملDescription of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5.
In order to generate a physical map of Arabidopsis thaliana chromosome 5, 142 molecular markers mapping chromosome 5 have been used in colony hybridization experiments with four Arabidopsis, ecotype Columbia, yeast artificial chromosome (YAC) libraries. This resulted in 634 YAC clones being anchored on chromosome 5. Southern blot analysis confirmed their positioning and provided data, which alo...
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ژورنال
عنوان ژورنال: Human Molecular Genetics
سال: 1994
ISSN: 0964-6906,1460-2083
DOI: 10.1093/hmg/3.4.629